Detection of hepatoma cells in peripheral blood of HCC patients by nested RT-PCR*
نویسندگان
چکیده
منابع مشابه
Detection of tumor cell contamination in peripheral blood by RT-PCR in gastrointestinal cancer patients.
We analyzed the peripheral blood of patients with gastrointestinal tract cancer at different stages to assess the presence of carcinoembryonic antigen (CEA) mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), which we used as an indicator for micrometastatic malignant cells. A total of 35 gastric, 24 colorectal, 4 esophageal and 4 biliary tract cancer patients and nine normal heal...
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Malignant melanoma (MM) prognosis has been related to tumour thickness and clinical stage and metastasis risk has been associated with presence of tumour cells in peripheral blood. The aim of this study was to determine the relationship between presence of tyrosinase in peripheral blood of MM patients and their clinical prognosis. Blood samples from 58 MM patients (stage I-IV) were analysed, us...
متن کاملDetection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis
Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...
متن کاملDetection of Mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer with nested RT-PCR.
OBJECTIVES The development and validation of a nested RT-PCR methodology for the detection of Mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer and evaluation of its prognostic significance. DESIGN AND METHODS Different combinations of specific primers were in silico designed and selected, so that false positive results due to geno...
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ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 1998
ISSN: 1007-9327
DOI: 10.3748/wjg.v4.i2.106